. 2015 Feb 3;5:8193. doi: 10.1038/srep08193

Table 2. Enzyme kinetic parameters of native (MeHNL), designed S-HNLs, and SABP2 toward racemic mandelonitrile^a.

Enzyme Name	k_cat	K_m	k_cat/K_m
	min ⁻¹	mM	min ⁻¹ mM ⁻¹
SABP2 (G12T/M239K)^b	0.9	13	0.072
HNL85	ND^c	ND	ND
HNL54	12 ± 0.6^d	3.2 ± 0.3	3.8
HNL30	156 ± 3	0.8 ± 0.1	195
MeHNL (WT)^a	2070	5.2	398

^aThe parameters are cited from literature values reported by Asano et al.²⁰.

^bThe parameters are cited from literature values reported by Padhi et al.²⁶.

^cND means “not determined”.

^dParameters were calculated by performing experiments in triplicate. The parameters are represented as averages ± standard deviations.

Table 2. Enzyme kinetic parameters of native (MeHNL), designed S-HNLs, and SABP2 toward racemic mandelonitrilea.