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. 2015 Feb 3;5:8193. doi: 10.1038/srep08193

Table 3. Steady-state parameters of three of the designed S-HNLs toward (R)- and (S)-mandelonitrile.

	HNL85			HNL54			HNL30
substrate	k_cat min⁻¹	K_m mM	k_cat/K_m mM⁻¹ min⁻¹	k_cat min⁻¹	K_m mM	k_cat/K_m mM⁻¹ min⁻¹	k_cat min⁻¹	K_m mM	k_cat/K_m mM⁻¹ min⁻¹
(S)-Man	ND	ND	ND	23.8	0.61	39.3	192	0.4	480
(R)-Man	ND^b	ND	ND	3.0	0.37	8.1	18.0	5.0	3.6
E value^a		ND			4.9 (S-selective)			133 (S-selective)

^aThe E value was calculated by following the equation shown in reference⁴⁵.

^bND means that the parameters could not be determined because of low enzymatic activity.