Figure 1. Stage 46 Xenopus laevis ‘three-eared’ frogs.

(a) Embryo with a transplanted third ear in the native orientation. (b) Embryo with a transplanted ear rotated 90 degrees from the native ear. (a’) Higher magnification of the natively-oriented transplanted ear and the right native ear in A. (b’) Higher magnification of the 90 degree rotated transplanted ear and the right native ear in B. (c) Three-dimensional reconstruction of a 90 degree rotated transplanted ear next to the native ear. Endolymphatic space is magenta, endolymphatic duct is cyan, and the hair cells are green. Native, unmanipulated ears are labeled ‘Ear’ and are circled with a black dotted line. Transplanted ears are indicated with a white arrow and are circled with a white dotted line. U, utricle; S, saccule. Blue and yellow arrows indicate ear orientation. Scale bar is 0.5 mm.