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. 2015 Feb 9;5:8345. doi: 10.1038/srep08345

Table 2. Oligos used for the construction of DNA fragments added to the integrative vector pID2GU (pINT:PDC6:UAS10:URA3:UAS7:PDC6) resulting in pID2G2SU equipped with I SceI recognition sites (pINT:PDC6:UAS10:I-SceI RS:URA3:I-SceI RS:UAS7:PDC6) and pID2G2LU equipped with Lox sites (pINT:PDC6:UAS10:loxP:URA3:loxP:UAS7:PDC6).

Plasmid Oligo name Nucleotide sequence (5′-3′)
pID2G2SU scesalxhoFW scesalxhoRV scespexbaFW scespexbaRV TCGACCTTAAGTTACGCTAGGGATAACAGGGTAATATAAC TCGAGTTATATTACCCTGTTATCCCTAGCGTAACTTAAGG CTAGTACGCTAGGGATAACAGGGTAATATAACGGAATTCT CTAGAGAATTCCGTTATATTACCCTGTTATCCCTAGCGTA
pID2G2LU loxsalxhoFW loxsalxhoRV loxspexbaFW loxspexbaRV TCGACATAACTTCGTATAGCATACATTATACGAAGTTATC TCGAGATAACTTCGTATAATGTATGCTATACGAAGTTATG CTAGTATAACTTCGTATAGCATACATTATACGAAGTTATT CTAGAATAACTTCGTATAATGTATGCTATACGAAGTTATA