Table 4. Overview of all the constructs mentioned in the experimental setup.
Abbreviation | Name | Description | Vector type, organism |
---|---|---|---|
pIDU | pSDM3585 | pINT:PDC6:URA3:PDC6 | Integrative vector, yeast |
pID2GU | pSDM3588 | pINT:PDC6:UAS10:URA3:UAS7:PDC6 | Integrative vector, yeast |
pID2G2LU | pSDM3586 | pINT:PDC6:UAS10:loxP:URA3:loxP:UAS7:PDC6 | Integrative vector, yeast |
pID2G2SU | pSDM3587 | pINT:PDC6:UAS10:I-SceI RS:URA3:I-SceI RS:UAS7:PDC6 | Integrative vector, yeast |
pRS425 VirD2-I-SceI | pSDM3592 | pRS425:pADH:NLS::virD2::I-SceI::virFCT | Expression vector, yeast |
pRS425 VirD2-Cre | pSDM3591 | pRS425:pADH:FLAG::NLS::virD2::Cre::virFCT | Expression vector, yeast |
pBFF I-SceI | pSDM8017 | pBFF:FLAG::NLS::I-SceI::virFCT | Expression vector, Agrobacterium |
pBFF VirD2-I-SceI | pSDM3884 | pBFF:FLAG::NLS::virD2::I-SceI::virFCT | Expression vector, Agrobacterium |
pBFF Cre | pSDM8019 | pBFF:FLAG::NLS::Cre::virFCT | Expression vector, Agrobacterium |
pBFF VirD2-Cre | pSDM8020 | pBFF:FLAG::NLS::virD2::Cre::virFCT | Expression vector, Agrobacterium |
p14-PDC6KX | pSDM3590 | pSDM14:rightborder:PDC6:KanMX:PDC6:leftborder | Binary vector, Agrobacterium |
P14-2GKX | pSDM3589 | pSDM14:rightborder:UAS7: KanMX:UAS10:leftborder | Binary vector, Agrobacterium |
pSDM8000 | pSDM14:rightborder:KanMX:leftborder16 | Binary vector, Agrobacterium |
For convenience, the constructs were given a short working name. All uninterrupted ORFs in the description are depicted in bold writing. The abbreviation “I-SceI-RS” indicates recognition sites for I-SceI.