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. 2015 Feb 9;5:8345. doi: 10.1038/srep08345

Table 4. Overview of all the constructs mentioned in the experimental setup.

Abbreviation Name Description Vector type, organism
pIDU pSDM3585 pINT:PDC6:URA3:PDC6 Integrative vector, yeast
pID2GU pSDM3588 pINT:PDC6:UAS10:URA3:UAS7:PDC6 Integrative vector, yeast
pID2G2LU pSDM3586 pINT:PDC6:UAS10:loxP:URA3:loxP:UAS7:PDC6 Integrative vector, yeast
pID2G2SU pSDM3587 pINT:PDC6:UAS10:I-SceI RS:URA3:I-SceI RS:UAS7:PDC6 Integrative vector, yeast
pRS425 VirD2-I-SceI pSDM3592 pRS425:pADH:NLS::virD2::I-SceI::virFCT Expression vector, yeast
pRS425 VirD2-Cre pSDM3591 pRS425:pADH:FLAG::NLS::virD2::Cre::virFCT Expression vector, yeast
pBFF I-SceI pSDM8017 pBFF:FLAG::NLS::I-SceI::virFCT Expression vector, Agrobacterium
pBFF VirD2-I-SceI pSDM3884 pBFF:FLAG::NLS::virD2::I-SceI::virFCT Expression vector, Agrobacterium
pBFF Cre pSDM8019 pBFF:FLAG::NLS::Cre::virFCT Expression vector, Agrobacterium
pBFF VirD2-Cre pSDM8020 pBFF:FLAG::NLS::virD2::Cre::virFCT Expression vector, Agrobacterium
p14-PDC6KX pSDM3590 pSDM14:rightborder:PDC6:KanMX:PDC6:leftborder Binary vector, Agrobacterium
P14-2GKX pSDM3589 pSDM14:rightborder:UAS7: KanMX:UAS10:leftborder Binary vector, Agrobacterium
  pSDM8000 pSDM14:rightborder:KanMX:leftborder16 Binary vector, Agrobacterium

For convenience, the constructs were given a short working name. All uninterrupted ORFs in the description are depicted in bold writing. The abbreviation “I-SceI-RS” indicates recognition sites for I-SceI.