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. 2015 Nov 17;10(11):e0143343. doi: 10.1371/journal.pone.0143343

Fig 4. Comparison between deletion gene copy number measurements made by SMART-ddPCR and MLPA.

Fig 4

Copy number measurements were available from ddPCR and MLPA assays for SNPs at the two deletion genes CDKN2A (SNP rs3731249) and IKZF1 (SNP rs4132601) in 27 and 75 tumor DNA samples respectively. (A) High correlation (R2 = 0.91) between the combined deletion gene copy number measurements made by ddPCR and MLPA. (B) Bland-Altman plot displaying the difference between measurements made in the same individual against their mean, as measured by two different methodologies (i.e. ddPCR and MLPA). There is very close agreement between the copy number measurements made by the two assays, as demonstrated by the narrow limits of agreement (-0.170 to 0.138) either side of the observed average agreement (-0.016).