Table 1. Primer parameters for SNPs identification and genotyping of BMP15.
Primer | Sequences (5′–3′) | Length of products/bp | Annealing temp (°C) | Usage |
---|---|---|---|---|
BMP15P1 | F: GGGACCTCTTTCTGCTTTACCR: CATCACCCATTGCCACCA | 406 | 58.4 | SNPs identification |
BMP15P2 | F: GGACAACAAGGGCAAGGGR: ATCGCATCGAGTGGAGACAA | 1096 | 59.4 | SNPs identification |
NC_006091.3:g.474A>G | F: GGGACCTCTTTCTGCTTTACCR: CATCACCCATTGCCACCA | 406 | 58.4 | Genotyping with HhaI |
NC_006091.3:g.594C>T | F: GGGTTTTAGCCCTGATCTTGCACTCR: ATCACCCATTGCCACCACCTTACCT | 517 | 58 | Genotyping with Alw21I |
NC_006091.3:g.1773T>C | F: TGAGCACCTTCTCCGTGTCAR: ATCCAATGGTCCCAACCC | 462 | 59.6 | Genotyping with MvaI |