Fig 2. Detection of heat shock protein 70 (HSP 70) in ileum by the comparison of a (A) 2D-DIGE image (CyDye 5) and (B) western blot.

2D-DIGE performed using 15 μg of protein labeled with each CyDye loaded on an 11 cm pH 3–10 strip for the first dimension and the second dimension on a 12.5% SDS-PAGE gel (acrylamide: N,N′-bis-methylene acrylamide 100:1, 0.1% SDS, 0.05% TEMED, 0.05% ammonium persulfate, and 0.5 M Tris–HCL, pH 8.8). Western blot protocols were followed after loading 40 μg of protein on a 7 cm 3–10 pH strip and running the second dimension on a 12.5% SDS-PAGE gel.