Table 3. Differentially abundant proteins between pair-fed thermal neutral (PFTN) and heat-stressed (HS) pigs at 12 h.

Spot ID	Protein a	Swiss-Prot name	Sequence Coverage	Pi (theo/exp)	MW (kDa) (theo/exp)	% Change b	P-value
515	Isocitrate Dehydrogenase [NADP], mitochondrial	IDH1_PIG	12%	8.74/9.10	48/	-70	<0.0001
158	Heat shock protein 90-α	HS90A_PIG	7%	4.93/4.94	85/	40	<0.0001
718	Heat shock protein β-1	HSPB1_PIG	24%	6.23/5.98	23/	20	0.0015
823	Peptidyl-prolyl cis-trans isomerase A	PPIA_PIG	14%	8.33/7.68	18/	-10	<0.0001
711	Peroxiredoxin-1	PRDX1_				-30	<0.0001
293	Stress-induced-phosphoprotein 1	STIP1_BOVIN	3%	6.08/	63/	32	0.0002
120	Endoplasmin precursor (HSP 90-β)	HS90B_MOUSE	9%	4.74/4.82	93/	19	0.0007
235	Heat shock protein 70 (HSP 70)	HS71A_MOUSE	8%	5.37/5.48	71/	20	<0.0001
192	Neurofibromin 1 (NF-1)	NF1_RAT	10%	6.94/7.10	32/	20	<0.0001
342	Immunoglobulin γ-chain	IGHG_PIG	6%	6.71/6.40	52/	18	0.0004
333	Heat shock protein 65	CH60_MOUSE	6%	5.91/	61/	10	0.0047
896	Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)	G3P_PIG	12%	6.90/8.57	36/	-8	0.0018
540	Fructose 1,6-Bisphosphate Aldolase	ALDOA_RABIT	12%	8.30/8.47	40/	-8	<0.0001
440	Alpha-enolase	ENO1_PIG	15%	6.37/7.01	48/	-10	<0.0001
739	Rho GDP inhibitor 1 (GDIR1)	GDIR_BOVIN	11%	5.12/5.03	24/	-14	<0.0001
812	Cofilin-1	COF1_PIG	13%	8.16/8.22	19/	-14	<0.0001
825	Peptidyl-prolyl cis-trans isomerase A	PPIA_PIG	19%	8.34/7.68	18/	-15	<0.0001

^a Trypsin used to digest all proteins

^b Positive values indicate an increase in HS pigs. Negative values indicate decrease in HS pigs.