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. 2015 Nov 17;11(11):e1005284. doi: 10.1371/journal.ppat.1005284

Fig 2. Activation of Rab11a GTPase in infected cells.

Fig 2

(A) HeLa cells were transfected with a plasmid expressing either GFP (lanes 1, 4, and 7), GFP-fused Rab11a constitutive active mutant (CA; lanes 2, 5, and 8), or GFP-fused Rab11a dominant negative mutant (DN; lanes 3, 6, and 9). At 24 h post transfection, cell lysates were prepared and subjected to GST pull-down assays with either GST (lanes 4–6) or GST-RBD (lanes 7–9). (B) At 8 h post infection (MOI = 10), mock-infected (lanes 1, 3, and 5) and infected HeLa cells (lanes 2, 4, and 6) were subjected to GST pull-down assays with either GST (lanes 3 and 4) or GST-RBD (lanes 5 and 6). Co-purified proteins were detected by western blotting assays with anti-Rab11a antibody. GST and GST-RBD were detected by CBB staining.