Table 3.
Summary of migration parameters in response to gradient fields of EGF and SDF-1.3
EGF Gradient Fields | SDF-1 Gradient Fields | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Migration Parameters | G1 | G2 | G3 | G4 | G5 | G1 | G2 | G3 | GG4 | G5 |
Fraction of motile cells (f) [%] | 28.3 | 18.6 | 11.9 | 10.3 | 3.7 | 18.2 | 20 | 13.8 | 8.7 | 6.5 |
Cell Directionality (D) [%] | 28.3 | 15 | 7 | 8.6 | 2.7 | 18.4 | 10.5 | 9.8 | 2.2 | 3.3 |
Average Cell Path Length (PL) in μm [Mean ± SD] | 281 ± 21 | 267 ± 40 | 226 ± 23 | 284 ± 28 | 389 ± 20 | 154 ± 34 | 114 ± 39 | 124 ± 42 | 94 ± 18 | 127 ± 37 |
Figure 1, Rico, CMB
Migratory parameters of MB-derived cells in response to gradient fields, G1-G5, generated by of 100ng/mL of EGF or SDF-1 along the μLane system, respectively. Fraction (f) and directionality (D) of motile cells shown as percentages of total cell numbers. Average maximum cell path lengths (PL) shown with mean and standard deviation. (ncells ~ 180 cells for each ligand).