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. Author manuscript; available in PMC: 2015 Nov 17.
Published in final edited form as: Dev Biol. 2008 Jan 3;315(2):317–330. doi: 10.1016/j.ydbio.2007.12.038

Figure 6.

Figure 6

Ripply genes modulate the RA response of Mesp genes. (A) Zebrafish embryos treated for 2 hours with RA and/or CHX as indicated and stained for the expression Ripply1 (top row) or Ripply2 (bottom row). Anterior is oriented up for zebrafish embryos. (B) Xenopus embryos treated for 1.5 hours with RA and/or CHX as indicated and stained for the expression of bowline (top row) or ledgerline (bottom row). Anterior is oriented to the left for frog embryos. (C - E) Zebrafish embryos injected with the indicated morpholino: R1 = anti-Ripply1; R2 = anti-Ripply2; R1+2 = both morpholinos. Injected embryos were treated for 4 hours with DMSO (carrier control) or with RA, and then stained for expression of C: mespb; D, mespa; or E, GFP. Anterior is up in all zebrafish panels. (F-I) Xenopus embryos injected with bowline (bln, F), ledgerline (ldg, G), both bln and ldg (H), or lacZ RNAs (I). Embryos were treated with DMSO or RA (top and bottom, respectively) and stained for localization of XMespβ transcripts. Within each boxed area, the two sides of a single embryo are shown. The uninjected side serves as an internal control and is always shown on the left side of the box.