Figure 1.

25(OH)D protects mice from NM-induced skin erosions. Circular mouse skin (50 mm²) biopsies were obtained 48 hours following topical NM exposure in presence and absence of 25(OH)D and compound 1400W for (a) representative images of NM-induced skin injury, (b) wound sizes measured relative to 50 mm² circular template (*p=0.042) (n=5) (c) histological images to assess NM-mediated skin necrosis and (d) detection of iNOS mRNA expression (n=9; P<0.003), (e) detection of activated macrophages by co-localization of iNOS (red) and F4/80+ (green) macrophages (indicated with arrows) using confocal microscopy. Mice were injected with clodronate liposomes or PBS liposomes following topical application of NM and 48 hours post exposure (f) imaged for wound regression, (g) histological assessment of skin injury and (h) detection of iNOS mRNA expression (n=4; P<0.043). All data presented as means±SEM. Scale Bar=100 μm.