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Surface-coated porous hydrogel studies and in vitro two-gene delivery system. Cumulative DNA release from HA porous hydrogels with surface-coated polyplexes with Col I treatment and in PBS (A). Cumulative (B) and per-day (C) 3-D transfection profile of cell-seeded surface-coated hydrogels over 14 d. 2-gene delivery transfection profile in porous hydrogels with surface-coated polyplexes containing pSEAP and encapsulated polyplexes containing pGLuc (D).
