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. Author manuscript; available in PMC: 2016 Nov 11.
Published in final edited form as: Cell Host Microbe. 2015 Oct 29;18(5):582–592. doi: 10.1016/j.chom.2015.10.007

Figure 3. Immunohistology of small intestine and colon during conventionalization.

Figure 3

Immunostaining of sections from distal small intestine (A) and distal colon (B) of Muc2 (green) with FISH detecting bacteria (red) and Hoechst counterstain of DNA (blue). Double arrows indicate the inner mucus layer. Inserts show the bacteria magnified in the boxed areas. Typically, the middle part of the inner mucus layer is not stained as well as Muc2 at other locations. Scale bars in A, upper panel and B, 50 μm; A, lower panel, 25 μm.