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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: J Invest Dermatol. 2015 Aug 28;135(12):3034–3040. doi: 10.1038/jid.2015.336

FIGURE 2.

FIGURE 2

PAF decreases the expression of DNMT 1 and 3b. (a) DNMT1 Protein expression in cPAF-treated HMC-1 cells was analyzed by immunoblotting; p84 is the loading control. (b) Time course of cPAF (10 μM)-induced DNMT1 depression. (c) Treating the cells with a PAF-receptor antagonist (AMT-491) blocks the cPAF-induced depression of DNMT1. (d) Time-course of cPAF-induced depression of DNMT3b expression. Effect of cPAF on DNMT1 and 3b mRNA expression as measured with RT-qPCR. Data represent the mean ± SEM (N=3), *p < 0.05 vs. control (Student's t-test).