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. Author manuscript; available in PMC: 2016 Nov 11.
Published in final edited form as: Cell Host Microbe. 2015 Nov 11;18(5):560–570. doi: 10.1016/j.chom.2015.10.012

Figure 3. CDK11 regulates levels of all HIV transcripts in 293T cells.

Figure 3

A. Schematic representation of HIV transcripts. The three main viral mRNA species are represented: unspliced (US), singly spliced (SS) and multiply spliced (MS) HIV transcripts. Arrows indicate the direction and location of primers.

B. CDK11 increases levels of all HIV transcripts. RT-qPCR of HIV RNA from cells that co-expressed f:CDK11 or plasmid vector and pNL4-3.Luc. Primers were designed to detect unspliced (US) genomic, singly spliced (SS) and multiply spliced (MS) HIV transcripts. Error bars represent mean ± SE, n = 3.

C. CDK11 depletion decreases HIV mRNA splicing. CDK11-depleted cells co-expressed the same plasmids as in panel B. Presented are RT-qPCR data with errors as in panel B. Western blots of CDK11 and actin are presented below the bar graph. See also Figure S3.