Skip to main content
. Author manuscript; available in PMC: 2016 Nov 11.
Published in final edited form as: Cell Host Microbe. 2015 Nov 11;18(5):560–570. doi: 10.1016/j.chom.2015.10.012

Figure 7. CDK11 increases length of polyA tails and stability of HIV transcripts in HeLa P4 cells.

Figure 7

A. Schematic representation of extreme 3’ RACE using the All Tail kit for polyA tail length, with indicated primer sets. Position 1 was assigned arbitrarily to the beginning of the forward primer (Fw). It is 9571 nucleotides removed from the 5’ end of the unsplised (US) genomic NL4.3.Luc transcript. Note that the ligated primer (in red) measures 20 nucleotides. Steps in the reaction are depicted in red. Rv, reverse primer.

B. Length of HIV polyA tails. HIV polyA tails in HeLa P4 cells co-expressing siCDK11, vector or f:CDK11 and pNL4-3.Luc were amplified by RT-qPCR and separated by a 2.5% agarose gel electophoresis.

C. CDK11 increases the stability of HIV transcripts. Measured was the stability of HIV genomic transcripts in cells co-expressing f:CDK11 and pNL4-3.Luc. After 24 hours, cells were treated with Actinomycin D and RNA was extracted at different time points. RT-qPCR was performed in duplicate. Error bars represent mean ± SE, n =3.