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. Author manuscript; available in PMC: 2015 Nov 17.
Published in final edited form as: Nat Genet. 2008 Nov 16;40(12):1454–1460. doi: 10.1038/ng.267

Figure 2.

Figure 2

XFoxJ1 morpholinos inhibits ciliogenesis in Xenopus skin cells.

(A-D) Xenopus embryos injected with both XFoxJ1 morpholinos, or a control morpholino were stained at stage 26 with ZO-1 (red) and anti-acetylated tubulin (green) to label cell borders and cilia, respectively (A,B), or were injected with RNAs encoding a membrane-localized RFP (red) and a GFP-centrin2 fusion protein (green), to label cell membranes and basal bodies, respectively (C,D). (E) Control or FoxJ1 morphants as above were co-injected with RNA encoding a GFP-centrin2 fusion protein (green), fixed at stage 26 and stained with rhodamine-phallodin (red) to label the apical actin network. Bottom panels show a 2μm Z-scan through the apical domain. Scale bars represent 20μm in A-B and 10μm in C-E.