Fig. 8.

Proteinuria and renal fibrosis data of SS control and SS-Adora2b mutant rats from the Ang II infusion study. The rats were infused with Ang II at a dose of 200 ng/kg/min for 4 weeks via subcutaneous implantation of osmotic minipumps. a Protein excretion rate and protein excretion rate to creatinine excretion rate ratio determined from 24-h urine collections at baseline (0) and then at 2 and 4 weeks after implantation of osmotic minipumps. b, c Renal fibrosis data. Kidneys were removed at the end of the study, and tissue sections were subjected to picrosirius red staining to evaluate the extent of interstitial fibrosis in cortical and medullary regions. Fibrosis was determined from fluorescent images. b Representative bright-field images and c collagen volume fraction data that was calculated using Image J software. Data are means ± SEM. n = 6–8 male rats/group