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Cardiac fibrosis of SS control and SS-Adora2b mutant rats from the Ang II infusion study. Hearts were removed at the end of the study and tissue sections were subjected to picrosirius red staining. Fibrosis was determined from fluorescent images. Shown are bright-field images (a) and the collagen volume fraction (b) that was calculated using Image J software. Data are means ± SEM. n = 6–8 male rats/group
