Fig. 3.
Effects of apyrase on electrically evoked [3H]ACh release from urothelium-denuded detrusor strips from control individuals (Control, a) and patients with outflow obstruction due to benign prostatic hyperplasia (BPH, b). Tritium outflow (ordinates) is expressed as a percentage of the total radioactivity present in the tissue at the beginning of the collection period (fractional release, %) (see, e.g., [21]). Abscissa indicates the times at which samples were collected. [3H]ACh release was elicited by electrical field stimulation (10 Hz, 200 pulses of 0.5 ms duration) twice, starting at 12th (S1) and 39th (S2) minutes after the end of washout (zero time). Apyrase (2 U mL−1, closed circles) was added to the incubation media 15 min before S2 (horizontal bar). The vertical bars represent SD of four different individuals. *P < 0.05 (unpaired Student’s t test with Welch’s correction) represents significant differences when compared to the situation without apyrase. Note that the spontaneous tritium outflow was not changed in the presence of apyrase