Fig. 6.
Effect of nucleotides on ATP in Capan-1 cells. a The extracellular ATP increase in Capan-1 cells after incubation with 0.1 (n = 5), 1 (n = 5) and 10 μM (n = 10) of UTP. b, c Stimulation of P2Y receptors with 10 μM of enzymatically stable agonists as follow: UTPγS (n = 7), UDP-glucose (n = 5), UDPβS (n = 9) and ADP (n = 4) did not increase the extracellular concentration of ATP in Capan-1 cells. In contrast triphosphates d, GTP and ITP (10 μM, n = 4, 7), show a similar ATP response as UTP. The left axis shows the ATP concentration values corrected per 106 cells in 1 ml. The arrows indicate the time of adding the stimulants