Fig. 7.
Effect of phosphate donors/acceptor on ATP generation. a Capan-1 cells generate extracellular ATP proportionally to the concentration of applied UTP from 0.001 to 100 μM in the presence of 10 μM ADP (n = 3). This effect was gradually decreased b, when the cells were stimulated with donor of phosphate groups, UTP (10 μM) and two different acceptors of phosphate groups ADP (1 μM) and increasing concentration of UDP (0.001–1,000 μM) (n = 3). c Extracellular ATP was generated in Capan-1 cells in presence of increasing concentration of acceptor ADP (0.001–100 μM) and 10 μM concentration of UTP. The left axis shows the ATP concentration values corrected per 106 cells in 1 ml. Results are given as mean net values ± SEM