Fig. 8.

The expression and activity of NDPK and AK1β in Capan-1 cells. a The activity of extracellular enzymes: nucleoside diphosphate kinase (NDPK, n = 3) and adenylate kinase (AK, n = 4) in Capan-1 cells. NDPK and AK were assayed by TLC with ATP and ADP (400 μM) and the labelled tracer [³H]ADP and AMP (400 μM), ATP (500 μM) and [³H]AMP as respective substrates. Enzymatic activities are presented as bar graph expressed as nanomoles of phosphorylated ³H-substrates by 10⁶ cells per 1 h. The left axis shows the ATP concentration values corrected per 10⁶ cells in 1 ml. Results are given as mean net values ± SEM. b The RT-PCR of extracellular membrane-bound adenylate kinase isoform 1β (AK1β) and NDPK domains (NME1, 2) in Capan-1 cells