FIG 3.

IL-6-mediated upregulation of CRP is dependent on HuR. (A) After IL-6 (50 ng/ml) treatment for 15 min, RIP analysis was performed by using HuR or IgG antibodies. CRP mRNA levels were measured by RT-qPCR analysis. (B and C) HepG2 cells were transfected with either control siRNA or HuR siRNA and treated with or without IL-6 for 24 h. The mRNA and protein levels of CRP and HuR were examined by RT-qPCR (B) and immunoblotting (C). CRP protein levels were quantified from immunoblots and normalized to values for the β-actin control. The histograms represent the means and standard errors of the means from three independent experiments. *, P < 0.05; **, P < 0.01 (by Student's t test).