FIG 1.

mRNA analysis of endometrial cancer specimens by real-time RT-PCR. (A and B) Twenty normal endometrial tissue specimens (NEM) and 37 primary HEC specimens were used to analyze their mRNA levels of BHLHE40 (A) and BHLHE41 (B). (C and D) BHLHE40/41 mRNA levels of the HEC group at the early stage (stage IA based on FIGO 2008 system criteria) were compared with those of the group at the advanced stage (more than stage IB). (E) The relationship between BHLHE40 and BHLHE 41 mRNA levels from 37 specimens was analyzed by Pearson's product-moment correlation coefficient. r values are correlation coefficients. The significance of these relationships was determined by the F-test. (F and G) The relationship between TWIST1 and BHLHE40 or TWIST1 and BHLHE41 mRNA levels was also analyzed by Pearson's product-moment correlation coefficient. A P value of <0.05 was considered significant. n.s., not significant.