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. 2015 Nov 17;35(24):4096–4109. doi: 10.1128/MCB.00678-15

FIG 4.

FIG 4

Impact of BHLHE40/41 in HEC cells analyzed by knockdown. (A) Protein expression levels of EMT markers in HHUA cells infected with lentivirus vectors to knock down BHLHE40, BHLHE41, or both were determined by immunoblotting. (B) A mRNA analysis of SNAI1, SNAI2, and TWIST1 in each knocked-down cell type was performed by real-time RT-PCR. (C) In vitro invasion of each knocked-down cell type. The right graph shows quantification data of the results. The scale bars represent 200 μm. shE40, shBHLHE40; shE41, shBHLHE41; *, P < 0.05; **, P < 0.01.