TABLE 1.
Primers used for the qRT-PCR analysis
| Target gene | Accession no. | Sequence |
Amplicon (bp) | |
|---|---|---|---|---|
| Forward primer | Reverse primer | |||
| BHLHE40 | NM_003670 | 5′-GACCGGATTAACGAGTGCAT-3′ | 5′-TGCTTTCACATGCTTCAAGG-3′ | 123 |
| BHLHE41 | NM_030762 | 5′-GCATGAAACGAGACGACACC-3′ | 5′-ATTTCAGATGTTCAGGCAGT-3′ | 126 |
| SNAI1 | NM_005985 | 5′-AAGGCCTTCTCTAGGCCCT-3′ | 5′-CGCAGGTTGGAGCGGTCAG-3′ | 113 |
| SNAI2 | NM_003068 | 5′-TTCGGACCCACACATTACCT-3′ | 5′-GCAGTGAGGGCAAGAAAAAG-3′ | 122 |
| TWIST1 | NM_000474 | 5′-CAGCTACGCCTTCTCGGTCT-3′ | 5′-CTGTCCATTTTCTCCTTCTCTGGA-3′ | 138 |
| ACTB | NM_001101 | 5′-TTGCCGACAGGATGCAGAAG-3′ | 5′-CAGCGAGGCCAGGATGGAGC-3′ | 122 |