TABLE 3.
Sequences of shRNA constructs
| shRNA name | Target | Target site | shRNA sequence (cloned into AgeI and EcoRI)a |
|---|---|---|---|
| Control shRNA (shCtrl) | None | CCTAAGGTTAAGTCGCCCTCG | 5′-accggtCCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGGTTTTTTTgaattc-3′ |
| shBHLHE40S1 (TRCN0000013249) | BHLHE40 exon 4 | GCACTAACAAACCTAATTGAT | 5′-accggtGCACTAACAAACCTAATTGATCTCGAGATCAATTAGGTTTGTTAGTGCTTTTTTgaattc-3′ |
| shBHLHE40S2 (TRCN0000232187) | BHLHE40 exon 4 | CATGTGAAAGCACTAACAAAC | 5′-accggtGCATGTGAAAGCACTAACAAACCTCGAGGTTTGTTAGTGCTTTCACATGCTTTTTTgaatttc-3′ |
| shBHLHE41S1 (TRCN0000086556) | BHLHE41 exon 2 | CTGGACTATTCCTCTTTGTAT | 5′-accggtGCTGGACTATTCCTCTTTGTATCTCGAGATACAAAGAGGAATAGTCCAGCTTTTTTgaattc-3′ |
| shBHLHE41S2 (TRCN0000016945) | BHLHE41 exon 1 | AGAGACAGTTACTGGAACATA | 5′-accggtAGAGACAGTTACTGGAACATACTCGAGTATGTTCCAGTAACTGTCTCTTTTTTTgaattc-3′ |
a
Cloning sites are shown in lowercase letters.