FIG 3.

The ACTACA submotif is sufficient to recruit the THAP11/HCFC1 subcomplex to ZNF143 SBS1 sites. (A) Diagram of mutations introduced for this set of experiments. The ACTACA sequence was inserted 2 bases 5′ of existing and ZNF143-bound SBS1 motifs (insA). The TCCCA sequence in the resulting construct was then mutated to destroy the ZNF143 binding site (insA/mutB). (B to D) Mutated and chromosomally integrated DNAJC10 (B), TFAM (C), and RNU6-2 (D) promoters were assayed by ChIP using THAP11, HCFC1, and ZNF143 antibodies. Bar graphs indicate means ± standard deviations of data from 3 replicate ChIP experiments.