FIG 8.

CRISPR-Cas9-mediated mutagenesis of DNAJC10 and TFAM promoters in SW620 cells. (A and B) Chromatograms obtained by Sanger sequencing of DNAJC10 and TFAM promoter PCR products (A) and TA-cloned TFAM (insA1 clone) promoter fragments representing individual alleles (B). (C) Genotypes of DNAJC10 and TFAM clones selected for further experiments. insA1 and insA2 represent two independent clonal isolates. HDR indicates alleles mutated by homology-directed repair. Indel indicates alleles mutated by nonhomologous end joining and containing random insertions/deletions.