FIG 1.

NGF upregulates TRPV2 in models of developing neurons. (A) E18 DRG neurons immunostained with antibodies against the thermoTRPV channels (TRPV1, TRPV2, TRPV3, and TRPV4) (green) and βIII-tubulin (red). Bar, 100 μm. (B) Western blot analysis of 50 μg of PC12 cell lysates using the indicated thermoTRPV antibodies plus phosphorylated Erk1/2 (pErk1/2), total Erk1/2, and β-actin. Cells were cultured in differentiation medium in the absence or presence of NGF (100 ng/ml) for 12 h. Since PC12 cells lacked TRPV1 immunoreactivity, mouse kidney lysate was loaded as a positive control.