FIG 7.

TRPV2 is phosphorylated by ERK. (A to C) E18 DRG neurons were cultured for 5 days in the presence of NGF and then fixed and immunostained for TRPV2 (green) and Rab7 (red) (A), TrkA (red) (B), or pErk1/2 (red) (C). The arrowheads point to regions of zoomed images represented in the bottom panels. Bars, 5 μm. (D) Recombinant MBP or TRPV2 (500 ng) was incubated in the absence or presence of recombinant active Erk2 (100 ng) and [γ-³²P]ATP, as indicated. Phosphorylation of MBP or TRPV2 was detected by autoradiography. The presence of recombinant proteins was confirmed by Coomassie staining.