FIG 5.

LSD1 regulates NANOS1 and NANOS3 expression in human cells. (A) Relative mRNA levels of the PUM complex component genes (PUM1 and PUM2 and NANOS1, -2, and -3) and bona fide LSD1 target (HES1) from MCF7 cells infected with two different shRNAs targeting LSD1 (LSD1-A2/A4), LSD2 (LSD2-B4/B5), and a scrambled control (Scr). (B) Relative RT-qPCR levels of the mammalian PUM complex member genes (PUM1, PUM2, NANOS1, NANOS2, and NANOS3) from MCF7 cells treated with DMSO or the LSD1 inhibitor TCP. (C) Relative LSD1, NANOS1, PUM1, and PUM2 expression in human normal bladder and tumor samples measured by RT-qPCR. GAPDH transcripts were used as a reference. Error bars indicate standard deviations. *, P < 0.05.