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. 2015 Nov 17;35(24):4199–4211. doi: 10.1128/MCB.00755-15

FIG 7.

FIG 7

Schematic representation of the interplay between the LSD1 family and the Pumilio complex. (A) Western blots of hemagglutinin (HA), LSD1, LSD2, and tubulin (TUB) from MCF7 cells transfected with HA-PUM expression constructs. (B) Western blots of LSD1, LSD2, PUM1, PUM2, and TUB from MCF7 cells infected with shRNAs targeting PUM1 (lanes 1-15 and 1-19), PUM2 (lanes 2-1 and 2-4), and a scrambled shRNA sequence (lane Scr). Relative protein level changes of LSD1 and LSD2 in cells depleted of PUM by shRNA are shown. (C) Schematic representation of the feedback loop between dLsd1 and the Pumilio complex in Drosophila melanogaster. (D) Schematic representation of the feedback loop between LSD1, LSD2, and the Pumilio complex in Homo sapiens.