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. 2015 Nov 14;5:64. doi: 10.1186/s13550-015-0138-7

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© Puri et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Sea urchin spine fiducial markers. a Example of a 5-μm-thick tissue section showing a sea urchin spine in the transverse plane in a haematoxylin and eosin (H&E)-stained histopathology slice at a spatial resolution of 1 μm/pixel. b 20-gauge spinal needle loaded with the sea urchin spine fiducial marker. The tip of the spine can be seen protruding from the needle (red arrow)