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. 2014 Jul 16;1:11. doi: 10.1186/s40634-014-0011-z

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© Jacinto-Tinajero et al.; licensee Springer 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Identification by western-blot of recombinant BMP-2, BMP-7 and TGF-β1 from Nicotiana benthamiana leaves. Crude extracts from plants containing each factor and obtained as described in the text were filtered on 0.2 μm membranes and loaded on a HisTrap HP column. Each factor was loaded separately. Fractions were dialyzed against PBS, loaded on a SDS-PAGE gel, transferred to nitrocellulose membranes and incubated with specific rabbit policlonal antibodies to confirm its identity. Molecular weight markers (kDa) are indicated on the left.