Fig. 6.

IL-17F triggers NFκB signaling in CLL, but not in healthy CD4⁻CD3⁺ (CD8⁺) cells. a CLL and healthy PBMCs were stimulated with IL-17F (20 ng/ml) or IL-17A (20 ng/ml) for 5 min. Phosphorylation of NFκB p105 was examined on CD4⁺CD3⁺ and CD4⁻CD3⁺ T cells by calculating the fold change in MFI for the cells in the IL-17F modulated as compared to the unmodulated (basal control) well for each donor sample. Each circular dot represents an individual CLL patient or healthy donor in which there were at least 100 gated cells. A similar analysis was performed for ERK (T202/Y204) and Akt (S473) (data not shown). Statistical significance between CLL and healthy was examined by unpaired two-sided Student’s t test (*p < 0.05)