Fig. 7.

Dysregulation of signaling by Th17-inducing factors may enhance Th17F cell differentiation. a, b CLL and healthy PBMCs were stimulated with IL-6 (50 ng/ml) (a) or IL-1β (100 ng/ml) (c) for 5 min. Phosphorylation of STAT1, STAT3, STAT4, or NF-kBp105 was examined on CD4⁺CD3⁺ and CD4⁻CD3⁺ T cells, by calculating the fold change in MFI for the cells in the modulated as compared to the unmodulated (basal control) wells for each donor sample. b For CD4⁺CD3⁺ T cells, the ratio of IL-6-induced pSTAT3 to pSTAT1, as well as the ratio of IL-6-induced pSTAT3 to pSTAT4, was calculated for each donor sample. Each circular dot represents an individual CLL patient or healthy donor in which there were at least 100 gated CD4⁺CD3⁺ or CD4⁻CD3⁺ cells. Statistical significance was examined by unpaired Student’s t test (*p < 0.05; **p < 0.005)