Fig. 2.

Low doses of poly(I:C) induce IL-36γ release in a dose and time-dependent manner. a Cells were treated with increasing concentrations of poly(I:C) for 96 h, and the conditioned medium analyzed by ELISA for accumulated IL-36γ. Bars show mean ± SD of IL-36γ per μg of cellular protein in the secreting monolayer (n = 8 experiments, *p < 0.001 relative to control cells treated with solvent). b Cells were treated with 1500 ng/ml of poly(I:C) and conditioned medium analyzed by ELISA at varying times. Bars show mean ± SD of IL-36γ levels, normalized per μg of cellular protein in the secreting monolayer (n = 4 experiments, *p < 0.001 compared to 0 time). c/d Cells were treated with 1500 ng/ml of poly(I:C) for 48 h, the medium removed, the cells washed, and medium replaced for an additional 48 h ± additional poly(I:C). Intracellular IL-36γ was measured by western blot (c) and IL-36γ levels in the conditioned medium measured by ELISA (d). Bars show mean ± SD of 4 experiments