Figure 8.

Deletion of PGC-1α only from parvalbumin interneurons recapitulates enhanced inhibition and increased I/E ratio seen in global PGC-1α^−/−. A, Efficiency and neuroanatomical specificity of recombination by PV-Cre was assessed with a tdTomato reporter mouse. Reporter signal (red) overlapped substantially with immunoreactivity for PV (green; colocalization appears yellow). Scale bar, 200 μm. B, q-RT-PCR revealed significant reductions in transcript expression of PV, Syt2, and Cplx1 in hippocampal homogenates from PGC-1α^fl/fl:PV-Cre (n = 12) compared with PGC-1α^WT:PV-Cre mice (n = 13). ***p < 0.0005, two-tailed, independent-samples t tests. C, The peak amplitude of disynaptic inhibition measured in CA1 pyramidal cells held at 0 mV is enhanced in PGC-1α^fl/fl:PV-Cre mice (n = 9, 14). D, The I/E ratio measured in CA1 pyramidal cells in response to Shaffer collateral stimulation is enhanced in PGC-1α^fl/fl:PV-Cre mice (n = 10, 7). *p < 0.05, Student's t test.