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. 2015 Nov 18;35(46):15403–15418. doi: 10.1523/JNEUROSCI.3165-15.2015

Figure 12.

Figure 12.

Comprehensive CST labeling in crym-GFP transgenic mice reveal extensive CST regeneration within the lesion site. Low-power photomicrographs of midsagittal sections from two crym-GFP ngr1−/− mice (A, C) show GFAP immunoreactivity (GFAP-IR) and reveal extensive astrogliosis at the DhX site (lesion epicenter is marked with an asterisk, inset compass in A shows orientation: D, dorsal; V, ventral; R, rostral; C, caudal). High-power photomicrographs from stippled box insets show GFAP-IR of quiescent astrocytes rostral to the lesion (A1i, C1i), dense hypertrophy of astrocytes in the lesion epicenter (A1ii, C1ii), and a decreasing hypertrophic profile caudal to the lesion site (A1iii, C1iii). Viewing sections under GFP illumination revealed robust detection of crym-GFP+ CST axons both rostral and caudal to the lesion site (B, D; crym-GFP, green; GFAP, red). High-power photomicrographs show extensive labeling of crym-GFP+ CST axons rostral to the lesion site (A2i, C2i), and regenerating into (A2ii, C2ii) and caudal to the lesion site (A2iii, C2iii) through areas of intense GFAP-IR (A3i-iii, C3i-iii, overlays; crym-GFP, green; GFAP, red). Scale bars: A, 1 mm; A1i, 100 μm.