Figure 4.

Semi-quantification of actin protein levels in the hippocampus of MWM-trained vs. untrained control CD1 mice. (A) Gel activation image of protein from hippocampal homogenates (15 μg) using UV illumination-based stain-free technology. (B) Image of nitrocellulose blot after protein transfer, indicating the total amount of protein in each lane that was used for normalization. (C) Representative Western blot detecting total actin (1:500) in hippocampal homogenates from MWM-trained and untrained control mice (n = 9–10). (D) Bar graph of densitometry values for actin, normalized to total protein and expressed as percentage change from control mean (100%) ± SEM, from hippocampal homogenates from untrained controls and MWM-trained mice. Error bars represent standard error; ^***p < 0.001.