Table 2.

Estimated Gibbs energy changes for the reduction of oxidized ferredoxin and NAD⁺ with dihydrogen (Equation 8).

In vitro system	Methanogenesis	Predicted dissolved H2 (M)	ΔG (kJ)
Batch	Non-inhibited	2.35 × 10−6	−60,151
Batch	100% inhibited	1.11 × 10−4	−77,112
Continuous	Non-inhibited	2.02 × 10−6	−59,366
Continuous	100% inhibited	2.54 × 10−5	−69,468