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Dnmt3a (a), Dnmt3b (b), Mecp2 (c), and Ezh2 (d) expression profile in melan-a, 4C3−, and 4C3+ before and after treatment with 5-aza-CdR. The protein expression was evaluated by Western blotting in melan-a, 4C3−, and 4C3+ cells that were untreated or treated with 10 μM 5-aza-CdR for 48 hours. The expression of β-actin was used for normalization. melan-a: nontumorigenic melan-a melanocyte lineage; 4C3−: nonmetastatic melanoma cell line; and 4C3+: metastatic melanoma cell line.
