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. 2015 Oct 8;5(5):690–701. doi: 10.1016/j.stemcr.2015.09.003

Figure 1.

Figure 1

In Vivo Imaging of Human HSPCs

CB mononuclear cells (CB MNCs) were flow sorted to purify Lin−CD34+CD38− (+/−) and Lin−CD34+CD38+ (+/+) populations and then labeled with dyes and injected IV in NSG recipients.

(A) Live distance measurements in μm of +/− and +/+ cells in the calvaria to bone at 16 hr and days 2, 3, and 4 post-transplantation.

(B) Live images from calvaria of mice transplanted with +/− (green, left) and +/+ (red, right) cells at 16 hr (top) and day 4 (bottom) after transplantation. Second harmonic signal generated by the bone is cyan. Scale bars represent 50 μm. N = 9 recipients of cells from pooled CB units (two recipients for 16 hr, two recipients for day 2, three recipients of day 3, three recipients of day 4). Each recipient received cells from approximately six to nine CB donors.