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. 2015 Oct 8;5(5):690–701. doi: 10.1016/j.stemcr.2015.09.003

Figure 5.

Figure 5

In Vivo Blocking of Integrins Reverses the Non-motile Phenotype of HSPCs

(A–E) In vivo treatment at day 4 post-transplantation of NSG mice with RAC1-GEF inhibitor NSC 23776 (3 mg/kg). Displacement (A), Speed (B), Straightness, (C) Distance to Bone, (D) and Sphericity (E) were measured from cells imaged in the calvaria, calculated before and after treatment. N = 2 recipients of six to nine pooled CB units for each recipient.

(F–J) In vivo treatment at day 4 post-transplantation of NSG mice with beta1 inhibitor-Bio 5192 (1 mg/kg) or CXCR4 antagonist-AMD3100 (1mg/kg). Displacement (F), speed (G), straightness (H), distance to bone (I), and sphericity (J) were measured from cells imaged in the calvaria and were calculated before and after treatment. N = 2 recipients (AMD3100) and 3 recipients (Bio 5192) of six to nine pooled CB units for each recipient. Error bars represent ± SD. ∗∗p < 0.01, ∗∗∗ p< 0.001; ∗∗∗∗p < 0.0001.