TABLE 2.
Inhibitory effect of alkyl phospholipid miltefosine and standard antifungals amphotericin B, fluconazole, and caspofungin on biofilm formation of Candida albicans clinical isolatesb
| Strain | BMIC (μg/ml) |
|||||||
|---|---|---|---|---|---|---|---|---|
| Miltefosine |
Amphotericin B |
Fluconazole |
Caspofungin |
|||||
| BMIC50 | BMIC90 | BMIC50 | BMIC90 | BMIC50 | BMIC90 | BMIC50 | BMIC90c | |
| 3034 | 2 | 4 | 2 | 8–16 | >1,000 | >1,000 | 0.25 | 0.2–0.5 |
| 4617 | 2 | 4 | 0.5 | 2 | 4 | 500 | 0.06–0.125 | 0.125 |
| 4639 | <2 | 4 | 0.25–0.5 | 2 | 8 | >1,000 | 0.03–0.06 | 0.06–0.12 |
| 5106 | 1–2 | 2 | 2 | 8 | >1,000 | >1,000 | 0.06–0.125 | 0.12–0.25 |
| 4380 | <2 | 4 | 2–4 | 8 | 2 | >1,000 | 0.125–0.25 | 0.25–16 |
| 2440 | <2 | 4 | 4 | 8 | >1,000 | >1,000 | 0.03 | 0.5 |
| 2307 | <2 | 2–4 | 0.5 | 4 | 2 | >1,000 | 0.125–0.25 | 0.25 |
| 412 | 2 | 4 | 1 | 8 | 2 | >1,000 | 0.06–0.125 | 0.125 |
| 1691 | 1–2 | 2 | 1 | 4 | 250 | >1,000 | 0.03–0.06 | 0.06–0.12 |
| 3731 | <2 | 2 | 0.5 | 4 | 125 | >1,000 | 0.06–0.125 | 0.12–0.25 |
| 6482 | 2 | 2–4 | 0.5–1 | 4 | 32 | >1,000 | 0.03–0.06 | 0.125 |
| 6191 | 2–4 | 4 | 1–2 | 2 | >1,000 | >1,000 | 0.25 | 0.5 |
| SC5314a | <2 | 4 | 0.5–1 | 2–4 | <2 | >1,000 | 0.06–0.125 | 0.125 |
a
Control strain.
b
Metabolic activity of biofilm cells was quantified using the XTT reduction assay after 24 h of incubation, and the inhibitory concentration was calculated in relation to control (not treated) biofilms.
c
All data in this column represent the paradoxical effect.