. 2015 Nov 17;59(12):7637–7649. doi: 10.1128/AAC.01746-15

TABLE 3.

Fitness cost of investigated Rif^r mutations

Mutant strain	No. of generations^a		Cost per generation^b	D_{0 − 1.0OD}^b^,^c	Relative fitness (g_R/g_S)^b
Mutant strain	g_S	g_R	Cost per generation^b	D_{0 − 1.0OD}^b^,^c	Relative fitness (g_R/g_S)^b
S549F	9.97	4.20	0.34 ± 0.12	−0.40 ± 0.17	0.42 ± 0.24
H553R	8.99	7.48	0.11 ± 0.02	−0.12 ± 0.03	0.83 ± 0.04
H553Y	9.02	9.1	−0.018 ± 0.04	0.018 ± 0.04	0.99 ± 0.006

The number of generations of the mutant strain (g_R) and of the wild-type strain (g_S) was calculated as described by Billington and coworkers (31). The values are the means from five independent experiments.

The values are presented as the means ± SDs from five independent experiments.

The difference in fitness (D_{0 − 1.0OD}) was estimated by direct competition against an equal number of CFU of the rifampin-susceptible wild-type strain.